034* Normal tissue 6 0 6 NF-��B inhibitor 0 *p < 0.05 Table 2 Comparing EGFR protein expression in neoplastic and paracancerous tissue Tissue type Number of cases EGFR Positive rate(%) P value positive negative Neoplastic tissue 50 23 27 46 0.020* Paracancerous tissue 7 0 7 0 *p < 0.05 Correlation between EGFR expression and clinical features The expression of EGFR in different subgroups were compared and summarized in Table 3. It shows that the difference of EGFR expression was only significant between the nodal positive and negative subgroups (56.4% vs.10%, p = 0.04). There

is no significant difference between age (60 vs. under 60 ys), selleck chemicals llc gender, adeno- vs. non-adenocarcinoma, the differentiation of tumor, and staging. Table 3 EGFR expression and clinical characteristics Clinical features EGFR Positive expression rate P value positive negative Ages 0.448 ≤60 18 14 43.80% >60 9 9 50% Sex 0.445 Male 16 15 40.50% Female 11 8 42.10% Pathologic type 0.543 Squamous carcinoma 13 8 40% Adencarcinoma 13 13 50% Mixed mTOR inhibition type 1 2 66.70% Tumor length 0.827 ≤3 cm 9 7 43.80% >3 cm 18 16 47.10% Level of Differentiation 0.474 Poor Differentiated 6 4 40% Moderate and Well Differentiated

21 19 47.50% TNM Stage 0.129 I-II 11 5 40% III 13 15 50.60% IV 3 3 50% Lymph node 0.006* N0 9 1 10% N1-3 17 22 56.40% *p < 0.05 EGFR expression and overall survival Cox proportional hazards analysis showed that EGFR protein positive expression independently

predicted patient survival, with RR of 2.311, p = 0.038, and 95% confidence interval (CI) of 1.049 – 5.095. The mean survival time for EGFR positive patients was 31 months, whereas the survival time was 48 months for patients with EGFR negative expression, with the latter significantly longer than the former (p = 0.008, Log Rank (Mantel-Cox))(Figure 2). Figure 2 Survival curves with different level of EGFR protein expression. The solid blue line indicates the survival for EGFR negative and the green line represents survival for EGFR positive expression subgroups. EGFR expression and outcome of radiotherapy In patients receiving post-operation thoracic irradiation, the mean survival time for EGFR positive patients (n = 15)was MycoClean Mycoplasma Removal Kit 25 months which was significantly shorter than that (48 months)for patients (n = 13)with EGFR negative expression (P = 0.004)(Figure 3). Figure 3 Survival curves based on EGFR expression in patients receiving thoracic irradiation. The solid blue line indicates the survival for EGFR negative and the green line represents survival for EGFR positive expression subgroups. COX-2 expression The positive rate of COX-2 protein expression in NSCLC tumor cells was 90%, which was significantly higher than that in normal tissue(p = 0.00) and paracancerous tissue (p = 0.00)(Figure 4, Tables 4 and 5). Figure 4 Immunohistochemical stain(×200)for COX-2 expression in (A) adenocarcinoma and (B) squamous carcinoma of the lung.