The 8 groups are: normal saline blank control group (blank group), TNBS model group (TNBS group), NF-κB missense oligonucleotide treatment
negative contrast I, II, III group (MSODN I, II, III click here group), NF-κB Decoy ODN treatment I, II, III group (Decoy ODN I, II, III group). 3. Observation indexes and test methods All groups of mice get executed by cervical vertebra dislocation one week after the last enema in order to get colon tissue. Use HE staining to evaluate the degree of inflammation of the colon tissue, VG staining to assess the degree of intestinal fibrosis. Use RT – PCR to detect the expression of IL- 1β, TNF – α and Col- III mRNA, immunohistochemistry to detect the expression of NF-κB and TGF-β1 protein of colon tissue. Results: 1. General situation and DAI score: The mice of blank group are normal diet, free movement, hair luster, no death during experimental; The mice of TNBS
group, MSODN I, II, III group are less diet, weight loss, exercise less, hair dull, diarrhea, stool occult blood, even the naked eye bloody stool within 3 days after enema weekly, four days later the symptoms gradually reduced, during the experiment, adverse symptoms mainly Angiogenesis inhibitor concentrate in the first three weeks, symptoms tend to be stable after three weeks, death mainly concentrate in the first three weeks; The mice of Decoy ODN I, II, III group can appear aforementioned symptoms, but to a lesser degree, adverse symptoms mainly concentrate in the first three days, then tend to be stable, during the experiment, MCE due to the different treatment of administration, adverse symptoms concentrate at different time, 3–4 weeks are more obvious, no death. The DAI score of each group: The DAI score of blank group is lower than other experimental groups and has statistical significance (P < 0.05); TNBS group and MSODN I, II, III group have no statistical significance (P > 0.05); The DAI scores of Decoy ODN
I, II, III group are lower than TNBS group, MSODN I, II, III group and have statistical significance (P < 0.05); Decoy ODN I, II, III group have statistical significance among three groups (P < 0.05). 2. General observation and pathological manifestation: The mice of blank group colon tissue appear reddish, no special change by macroscopic observation. The mice of TNBS group, MSODN I, II, III group can observe that visible deformation of colon bowel, thicken adhesion of bowel wall, and hyperemia, edema, erosion, ulcer, even nodule; The mice of Decoy ODN I, II, III group can appear aforementioned changes, but to a lesser degree, and no visible deformation of colon bowel, thicken adhesion of bowel wall, the degree of Decoy ODN III group is the lightest.