This constituted the VPC familiarization phase. Infants were then tested using the VPC at three delays: (1) immediately following familiarization (“Imm”), (2) two minutes following familiarization (“2 min”), and (3) 1 day after familiarization (“Day 2”). For each of these three VPC tests, infants were shown the familiar face next to a novel face for a total of 20 sec and the left or right position of the faces switched sides after 10 sec. At each VPC comparison

test, infants saw a unique face paired with the familiarization selleck kinase inhibitor face. The Day 2 visit began with the final portion of the eye-tracking experiment and concluded with the ERP paradigm. Infants were again calibrated to ensure successful gaze tracking with the Tobii monitor and then presented with the third and final VPC test comparison (Day 2). After the eye-tracking portion of the experiment, the ERP task began. Before fitting the child with the HCGSN, infants were familiarized to a new face. This face was presented 20 times for 500 ms in the center of the screen with a variable intertrial interval of no less than 1,500 ms.

EEG was then recorded as infants saw this newly familiarized face (“recent familiar”), the VPC familiarization face from Day 1 and Day 2 (“VPC”) and a third never-before-seen face (“novel”) presented in a semirandomized order such that for every three stimuli presented, these three faces each appeared once (so they were randomized within every set of three). This ensured GPCR Compound Library cell line an even number of presentations of each of the three stimuli. Stimuli were counterbalanced across participants, such that the “VPC” face for one set of infants would serve as the “recent familiar”

face for a second set of infants and the “novel” face for a third set of infants. From a separate room, an experimenter observed infant’s eye movements and attentiveness through a video camera mounted on top of the experimental monitor. Stimulus presentation was initiated only when the child was attending to the screen, and any trial where an infant’s attention shifted during image presentation was flagged and removed from later analysis. Images were presented until infants saw a maximum of 126 trials or until the infant became too fussy to continue. Nutlin-3 price Gaze data were collected at a sampling rate of 60 Hz throughout the testing session. Before the eye-tracking data were exported from the Tobii Studio program, areas of interest (AOIs) were drawn onto the stimuli, enabling the subsequent analysis of gaze data within these particular AOIs. A single AOI was created for each picture that encompassed the face and gray background and was labeled as familiar face or unfamiliar face. Each participant’s eye-tracking data were exported from Tobii Studio, with time samples identified in which gaze fell within one of the faces. These exported data files were run through a custom-made Python script (Python Programming Language; www.python.