They were kept frozen for 40 days, and then a histological study was carried out. Another 10 tendon samples were analyzed while still “fresh”.\n\nRESULTS: There was no histological difference between the fresh and frozen samples in SN-38 relation to seven variables.\n\nCONCLUSIONS: Semitendinous muscle tendon allografts can be submitted to cryopreservation at -80 degrees C without suffering histological modifications.”
“AIM: To study the tear film stability after lamellar keratoplasty\n\nMETHODS: Five female and eight male patients with lamellar keratoconus, aged from 18 to 32, were involved. After lamellar keratoplasty, Schirmer I test (S I t), tear break-up time (BUT)

test, fluorescein staining test were used to judge the effect of the surgery at different time point.\n\nRESULTS: The S 1 t were greatly increased in 7 clays post operation (11.86+/-2.28 -25.14+/-1.97, 19.86+/-1.61) (P<0.05), there is no significant difference between 2nd month, 3rd month post-operative and

pre-operation(11.86+/- 2.28 – 14.57+/-1.48, 8.14+/-0.86) (P >0.05). The mean break-up time decreased in 7 GW4869 cost days post operation (5.00+/-1.31 -2.71+/- 0.18, 2.57+/- 0.20, 2.71+/- 0.36, 2.43+/- 0.20) (P <0.05). The mean scores of fluorescence increased post-operatively (0.14+/- 0.14 – 8.00+/- 0.00, 8.00+/- 0.00, 8.00+/- 0.00, 7.57+/- 0.20) (P<0.01).\n\nCONCLUSION: Lamellar keratoplaty influence the tear film stability, artificial tears and improving corneal epithelium cured medicine should be used after surgery.”
“During July 2012, a severe unusual disease symptom was observed on young shoots on apricot (Prunus armeniaca 10/13 hybrid) in the city of Pomaz, near Budapest. The naturally infected shoots

showed typical symptoms of SRT2104 purchase fire blight including terminal shoots with brown to black necrotic lesions. Symptoms were the same as fire blight symptoms reported from other hosts and locations. The first occurrence of fire blight on an apricot tree in Europe was recorded in Czech Republic in 2011. Samples of the leaves and shoots with symptoms were macerated and spread on King’s medium B. After 24 hours of incubation at 26 C, bacteria morphologically similar to E. amylovora were detected. Isolate induced hypersensitive reaction on tobacco (Nicotiana tabacum L. ‘White Burley’) leaves. Biochemical test was also used for identification, and the result of API 20E kit (Biomerieux, Marcy l’Etoile, France), demonstrate that the bacterium belongs CO Enterobacteriaceae family. A pathogenicity tests were positive on young apricot shoots and immature fruits. For molecular identification of the pathogen the 16S rDNA region was amplified from isolate Ea-ApricotPol with a general bacterial primer pair (631 forward and 1389r reverse). The PCR products were cloned into a pGEM T-Easy plasmid vector (Promega, Madison, WI USA) and were transformed into Escherichta coil DH5 alpha cells.