Here, we tested this assumption for ichthyotoxic flagellates of the genus Pseudochattonella (Dictyochophyceae) under different light, temperature, salinity, and nutrient conditions. Our results show changes in cellular RNA contents of nearly one order of magnitude depending on the condition and also the time of exposure, rendering GS-1101 solubility dmso it difficult to anticipate per-cell RNA yields even if environmental conditions are known. However, cellular RNA content was positively correlated with cell size and growth rate across our experiments, and total RNA was comparable to cell
number as a predictor for total biovolume. These results demonstrate the importance of considering the variability of RNA levels for comparisons with cell counts and provide a valuable aid for the interpretation of data from RNA-based detection methods. “
“Temperature is one of the major environmental factors that affect the distribution, growth rate, and life Lenvatinib price cycle of intertidal organisms, including red algae. In an effort to identify the genes involved in the high-temperature tolerance of Porphyra, we generated 3,979 expression sequence tags (ESTs) from gametophyte thalli of P. seriata Kjellm. under normal growth conditions and high-temperature
conditions. A comparison of the ESTs from two cDNA libraries allowed us to identify the high temperature response (HTR) genes, which are induced or up-regulated as the result of high-temperature treatment. Among the HTRs, HTR2 encodes for a small polypeptide consisting of 144 amino acids, which is a noble nuclear protein. Chlamydomonas expressing the Porphyra HTR2 gene shows higher survival and growth rates than the wild-type strain after high-temperature treatment. These results suggest that HTR2 may be relevant to the tolerance of high-temperature stress conditions, and this Porphyra EST data set will provide important genetic information for studies of the molecular
basis of high-temperature tolerance in marine algae, as well as in Porphyra. “
“Astaxanthin-rich oil globules in Haematococcus pluvialis display rapid light-induced peripheral 上海皓元医药股份有限公司 migration that is unique to this organism and serves to protect the photosynthetic system from excessive light. We observed rapid light-induced peripheral migration that is associated with chlorophyll fluorescence quenching, whereas the recovery was slow. A simple assay to follow globule migration, based on chlorophyll fluorescence level has been developed. Globule migration was induced by high intensity blue light, but not by high intensity red light. The electron transport inhibitor dichlorophenyl-dimethylurea did not inhibit globule migration, whereas the quinone analog (dibromo-methyl-isopropylbenzoquinone), induced globule migration even at low light. Actin microfilament-directed toxins, such as cytochalasin B and latrunculin A, inhibited the light-induced globule migration, whereas toxins against microtubules were ineffective.