“
“Background: Low molecular weight thiols (cysteine, cysteinylglycine, glutathione [GSH] and homocysteine) are
important intermediates in different metabolic pathways. Glutathione has a relevant role as an antioxidant in detoxification of toxic compounds and xenobiotics, and homocysteine represents a risk factor for cardiovascular, neurological, and congenital diseases. KPT-8602 Homocysteine and GSH are metabolically related in a pathway including cysteine and cysteinylglycine as intermediates. For these reasons, determination of homocysteine and related thiols is of great importance in the diagnosis of several diseases.
Methods: The measurement of these sulphur compounds can be performed by using different methods, such as liquid or gas chromatography/mass spectrometry, high-performance liquid chromatography (HPLC), or capillary electrophoresis.
Results: This study describes an HPLC method coupled with fluorimetric detection for the simultaneous determination of cysteine, cysteinylglycine, GSH, and homocysteine in different biological fluids (blood, saliva, urine,
NVP-BSK805 price and cerebrospinal fluid [CSF]).
Conclusion: The comparison of the results obtained by other authors, as well as the method validation and the analytical costs, indicate that this HPLC method is particularly suitable for routine measurement of thiols in different body fluids.”
“Objectives: The purposes of this study are to validate the indicator of Oral Health Impact Profile for edentulous patients (OHIP-20sp) in the Spanish population and to analyze the factorial construct of the prosthetic well-being.
Study Desing: A total of twenty-one
(n=21) edentulous patients wearing mandibular implant-overdentures on Locator (R) (LO) and twenty (n=20) with complete dentures (CD) were retrospectively evaluated in this study. All participants were recruited consecutively and were treated in the previous academic year 2009-2010 by professors of the University of Salamanca. Reliability analyses and validity tests were performed in order to evaluate the Barasertib nmr psychometric properties of OHIP-20sp employing two different total score methods (additional and simple count). A retrospective evaluation of the impact of the prosthetic treatment was captured with an evaluative instrument derived from OHIP-20, and named POST-OHIP-13.
Results: The reliability coefficient (Cronbach’s alpha=0.91) has shown a high internal consistency. Item-total correlations coefficients ranged from 0.46 and 0.81. Five factors, named as disability, functional comfort, psychosocial impact, pain-discomfort and functional limitations were identified as principal components of the construct, explaining almost 85% of the variance. The 48% of the sample felt at least one impact in an occasional or more frequently manner (generally food packing).