Whereas semi-quantitive method reported the most frequently isolated Selonsertib cost bacteria from intravascular catheters

as coagulase-negative staphylococci and staphylococcus aureus [16, 40], our molecular data analysis from 16S rRNA gene clone sequences presented Stenotrophomonas maltophilia as the predominant bacteria. There are several reports of discrepancies between culture-dependent and culture-independent approaches for bacterial community studies [29, 41, 42]. Culture dependent methods bias bacteria who favour the growth media and grow fast under standard laboratory conditions. In addition, some bacterial species may compete with others for nutrients or they may even inhibit other bacteria from growing [20, 41, 43]. Unlike the semi-quantitive method, which only examines bacteria on outer surfaces of catheters, the molecular method used here enables assessing bacteria on both inner and outer surfaces of catheters. Together these factors might help explain variations LCZ696 cost of the bacterial community examined by these two methods. Compared to culture-dependent methods, culture-independent methods provide more comprehensive information on the bacterial community. The knowledge gained from

this study may be a beginning step in improved understanding of pathogenesis and infection risks for critically ill patients with intravascular catheters. GDC941 Replication of this study in other settings, Branched chain aminotransferase as well as exploring the relationship between type and timing of commencement for antibiotic therapy, and diagnostic results, are important areas for future research. Conclusions This study

of critically ill patients with suspected CRI, has demonstrated that both colonised and uncolonised ACs examined by molecular method have an average of 20 OTUs per catheter, most of which are not isolated by the semi-quantitative method. Overall there were 79 OTUs in the two sets of samples which comprised 51 OTUs for colonised ACs and 44 OTUs uncolonised ACs. Of the 79 OTUs identified in the two sets of samples, 40 were identified in both groups. Statistically there was no significant difference in bacterial composition between uncolonised and colonised ACs, as confirmed by the results of t-test of taxonomic group distribution, the OTU distribution, and diversity indices. Taken together, this study suggests that in vascular devices removed for suspicion of CRI and analysed using semi-quantitative method, a negative culture result may not be indicative of non infective catheters. Moreover, these culture negative catheters may at times be a significant source of sepsis in critically ill patients. Whilst the clinical significance of these findings requires further study before any such conclusions may be drawn, the results suggest a need for the development of new methods that more accurately determine the presence of pathogens on intravascular devices.