We have a hypothesis about how do CCR7 trigger PI3K/Akt signal pathway. The expression of lymph node chemokine in T-NHL could cause the upregulation of chemokine receptors. The interaction between chemokines and their receptors may then activate the Akt protein by peroxodiphosphoric acid, followed by the activation of the PI3K/Akt signal pathway, which can promote tumor cell proliferation and invasion. This result provides a theoretical foundation for the targeting of CCR7 and the PI3K/Akt signal pathway with antibodies for the treatment of
T-NHL. However, further studies on the concrete mechanism of activation of this pathway and its downstream genes are still needed. In this study, we also detected expression of MMP-9 and MMP-2. MMP is a matrix metalloproteinase that breaks down and destroy Type IV and Type V collagen, as well as gelatin Mocetinostat research buy in the extracellular matrix, and then promote tumor metastasis. CCR7 expression in T-NHL was directly correlated with MMP9 expression. High MMP-9 expression has previously been reported in non-Hodgkin’s lymphoma [28, 29], which can influence the biological behavior and clinical progression Selleck PXD101 of tumor. For T-NHL, a report in an animal experiment found that the high expression of MMP-9 is correlated with liver metastasis [30]. The high expression of
MMP-9 is also associated with bad prognosis.
The relationship between CCR7 and MMP-9 suggests that these two factors may Vildagliptin enhance each other and promote tumor dissemination synergistically. However, the function of MMP-2 in T-NHL metastasis is still unclear. Conclusions Higher CCR7 expression in T-NHL cells is significantly associated with lymphatic and distant dissemination in patients, as well as with migratory and invasive phenotypes in vitro. Our study suggested that CCR7 plays an important role in the progression of T-NHL. The possible mechanism is via the PI3K/Akt signal pathway. Further studies are needed to evaluate the inhibition of metastatic growth through blocking CCR7 and PI3K/Akt signal pathway. Acknowledgements This work was partly supported by a grant from key project of the National Natural Science Foundation of China (No. 30830049), the International cooperation of the Tianjin Natural Science Foundation (CMM-Tianjin, No. 09ZCZDSF04400), Key project of the Tianjin Natural Science Foundation (No. 09JCYBJC12100) References 1. Arya M, Patel HR, Williamson M: Chemokines: key players in cancer. Curr Med Res Opin 2003, 19:557–64.PubMedCrossRef 2. Yoshida R, Nagira M, Kitaura M, Imagawa N, Imai T, Yoshie O: Secondary lymphoid tissue chemokine is a functional ligand for the CC chemokine receptor CCR7. Biol Chem 1998,273(12):7118–7122.CrossRef 3.