These findings provide a conceptual framework that interneurons serve as a key regulator of initiating sequential spike activity. “
“Dendritic spines form the postsynaptic half of the synapse but how they form during CNS development remains uncertain, as are the factors that promote their morphological and physiological maturation. One hypothesis posits that filopodia, long motile dendritic processes that are present prior to spine formation, are the precursors to spines. Another hypothesis posits that they form directly from the dendritic shaft. We used microphotolysis of caged glutamate to

stimulate individual dendritic processes SAHA HDAC in young hippocampal slice cultures while recording their morphological and physiological responses. We observed that brief trains of stimuli delivered to immature selleck kinase inhibitor processes triggered morphological changes within minutes that resulted, in about half of experiments, in a more mature, spine-like appearance such as decreased spine

neck length and increased spine head width. We also observed that glutamate-induced inward currents elicited from immature processes were mostly or entirely mediated by NMDARs, whereas responses in those processes with a more mature morphology, regardless of actual developmental age, were mediated by both AMPARs and NMDARs. Consistent with this observation, glutamate-induced morphological changes were largely, but not entirely, prevented by blocking NMDARs. Our observations thus favor a model in which filopodia in the developing nervous system sense and respond to release of glutamate from developing axons, resulting in physiological and morphological maturation. “
“Multivariate pattern classification analysis

(MVPA) has been applied to functional magnetic resonance imaging (fMRI) data to decode brain states from spatially distributed activation patterns. Decoding upper limb movements from non-invasively recorded human brain activation is crucial for implementing a brain–machine interface that directly harnesses an individual’s thoughts to control external devices or computers. The aim of this study was to decode the individual finger movements from fMRI single-trial oxyclozanide data. Thirteen healthy human subjects participated in a visually cued delayed finger movement task, and only one slight button press was performed in each trial. Using MVPA, the decoding accuracy (DA) was computed separately for the different motor-related regions of interest. For the construction of feature vectors, the feature vectors from two successive volumes in the image series for a trial were concatenated. With these spatial–temporal feature vectors, we obtained a 63.1% average DA (84.7% for the best subject) for the contralateral primary somatosensory cortex and a 46.0% average DA (71.0% for the best subject) for the contralateral primary motor cortex; both of these values were significantly above the chance level (20%).