The interactions of H. pylori VacA cytotoxin and ANTs were detected by yeast two-hybrid and co-immunoprecipitation assays. Results: Transfection of H. pylori VacA p37 increased the mRNA and protein expression of ANT1 and ANT3, but not ANT2. Moreover, VacA p37 up-regulated Bax expression of but down-regulated Bcl expression of -2 at both mRNA and protein levels in AGS cells. Yeast two-hybrid and

co-immunoprecipitation assays did not show any protein interaction between H. pylori VacA p37 and ANTs. Conclusion: H. pylori selleck products VacA induces mitochondrion-mediated apoptosis of AGS cells in an ANT isoform-specific manner, and the Bcl-family is involved in this process. However, H. pylori VacA appears not to directly interact with ANTs to mediate mitochondrion-mediated apoptosis. Key Word(s): 1. Helicobacter pylori; 2. VacA cytotoxin; 3. ANTs; 4. Yeast two-hybrid; Presenting Author: FENGPING ZHENG Additional Authors: XIANYI LIN, LI TAO, YUNWEI GUO Corresponding Author: FENGPING ZHENG Affiliations: The Third Affiliated Hospital of Sun Yat-Sen University Objective: Increasing

resistance against H. pylori has resulted in reduced eradication rates. The efficacy and tolerability of a second-line furazolidone-containing triple regimen (PPI-amoxicillin-furazolidone) in the eradication of H. pylori was evaluated. Methods: 217 Patients with a diagnosis of HP-positive confirmed using endoscopy Belnacasan research buy or C13 urea breath testing were eligible for inclusion in this study. All patients underwent a washout period of 6 weeks from any prior antibiotic or PPI usage. Patients were randomized to either amoxicillin, furazolidone and esomeprazole therapy for 10 days, including amoxicillin 1000 mg twice daily with

meals, furazolidone 100 mg twice daily with meals, and esomeprazole 20 mg twice daily with meals, or esomeprazole, amoxicillin, and clarithromycin standard therapy for 10 days, which included esomeprazole 20 mg, amoxicillin 1 g with breakfast and dinner, and clarithromycin 500 mg with breakfast and dinner. HP eradication was confirmed selleck chemicals by C13 urea breath testing at least 4 weeks after cessation of therapy. Results: Intention-to-treat analysis revealed significant differences (P < 0.05) in the eradication rates of the amoxicillin, furazolidone, esomeprazole 10 days therapies were 82.9% (87/105) compared with those receiving standard treatment eradication rates were 73.2% (82/112). There were no differences in adverse effects between the groups. Conclusion: Amoxicillin, furazolidone and esomeprazole 10 days therapies were prior to standard treatment eradication. A large randomized controlled trial is warranted to further evaluate the efficacy of this regimen. Key Word(s): 1. Eradication; 2. Helicobacter Pylori; 3.