PLoS One 2010, 5:e8619

PLoS One 2010, 5:e8619.PubMedCrossRef 32. Lenhart TR, Akins DR: Borrelia burgdorferi locus BB0795 encodes a BamA orthologue required for growth and efficient localization of outer membrane proteins. Mol Microbiol 2010, 75:692–795.PubMedCrossRef TPCA-1 ic50 33. Elias AF, Stewart PE, Grimm D, Caimano MJ, Eggers CH, Tilly K, Bono JL, Akins DR, Radolf JD, Schwan TG, Rosa P: Clonal polymorphism of Borrelia burgdorferi strain B31 MI: implications for mutagenesis in an infectious strain background. Infect Immun 2002, 70:2139–2150.PubMedCrossRef 34. Gilbert MA, Morton EA, Bundle SF, Samuels DS: Artificial regulation of ospC expression in Borrelia burgdorferi . Mol Microbiol 2007, 63:1259–1273.PubMedCrossRef

35. Barbour AG: Isolation and cultivation of Lyme disease spirochetes. Yale J Biol Med 1984, 57:521–525.PubMed 36. Skare JT, Shang ES, Foley DM, Blanco DR, Champion CI, Mirzabekov T, Sokolov Y, Kagan BL, Miller JM, Lovett MA: Virulent strain associated outer membrane proteins of Borrelia burgdorferi . J Clin Invest 1995, 96:2380–2392.PubMedCrossRef 37. Promnares K, Kumar M, Shroder DY, Zhang X,

Anderson JF, Pal U: Borrelia burgdorferi small lipoprotein Lp6.6 is a member of multiple protein complexes in the outer membrane and facilitates pathogen transmission from ticks to mice. Mol Microbiol 2009, 74:112–125.PubMedCrossRef 38. Schagger H, von Jagow G: Blue native electrophoresis for isolation of membrane protein complexes in enzymatically active form. Anal Biochem 1991, 199:223–231.PubMedCrossRef 39. Brooks CS, BTK inhibitors high throughput screening Vuppala SR, Jett AM, Akins DR: Identification selleckchem of Borrelia burgdorferi outer surface PJ34 HCl proteins. Infect Immun 2006, 74:296–304.PubMedCrossRef 40. Kenedy MR, Vuppala SR, Siegel C, Kraiczy P, Akins DR: CspA-mediated binding of human factor H inhibits complement deposition and confers serum resistance in Borrelia burgdorferi . Infect Immun 2009, 77:2773–2782.PubMedCrossRef 41. Kyte J, Doolittle RF: A simple method

for displaying the hydropathic character of a protein. J Mol Biol 1982, 157:105–132.PubMedCrossRef 42. Bendtsen JD, Nielsen H, von Heijne G, Brunak S: Improved prediction of signal peptides: SignalP 3.0. J Mol Biol 2004, 340:783–795.PubMedCrossRef 43. Nielsen H, Engelbrecht J, Brunak S, von HG: Identification of prokaryotic and eukaryotic signal peptides and prediction of their cleavage sites. Protein Eng 1997, 10:1–6.PubMedCrossRef 44. Juncker AS, Willenbrock H, von Heijne G, Brunak S, Nielsen H, Krogh A: Prediction of lipoprotein signal peptides in Gram-negative bacteria. Protein Sci 2003, 12:1652–1662.PubMedCrossRef 45. Waterhouse AM, Procter JB, Martin DMA, Clamp M, Barton GJ: Jalview Version 2-a multiple sequence alignment editor and analysis workbench. Bioinformatics 2009, 25:1189–1191.PubMedCrossRef 46. Akins DR, Purcell BK, Mitra M, Norgard MV, Radolf JD: Lipid modification of the 17-kilodalton membrane immunogen of Treponema pallidum determines macrophage activation as well as amphiphilicity.

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