Numbers of protease MK-0518 price producing isolates (P) versus non producers (NP) were compared using Fisher’s exact test. A P value < 0.05 was considered statistically significant. I = Italy, NZ = New Zealand, RA = Argentina, H = Hungary. Univariate regression was applied to determine whether an association existed between the expression of the two virulence factors studied. As shown in Figure 5, a negative correlation between biofilm production and proteinase secretion by the C. parapsilosis isolates was observed (r = -0.483, P

< 0.0001). Figure 5 Correlation between biofilm and proteinase production. Negative correlation between biofilm production and proteinase secretion in Candida parapsilosis isolates (n = 62), as revealed by univariate regression analysis. Pearson's correlation coefficient (r) and P-value are indicated. Discussion To date, no significant sequence variation has been described

for Candida parapsilosis [30]. Therefore, this study was designed to provide further information on genotypic and phenotypic properties of this opportunistic fungal pathogen. To evaluate the effect of different environments upon genetic MK-2206 ic50 variability C. parapsilosis click here isolates were selected to be representative of different geographical regions (Italy, Hungary, New Zealand, Argentina) and of different anatomical sites (blood, cerebrospinal fluid, mucosa, nail etc.). The EcoRI/HindIII enzyme combination used in the AFLP protocol was expected to produce a higher number of polymorphic bands since in C. metapsilosis band homoplasy was reduced with this combination and the average fragment length was larger than the one obtained with EcoRI/MseI [17]. Indeed the EcoRI/HindIII enzyme combination confirmed its higher discriminative power for C. parapsilosis and led to the identification of 20.7% of polymorphic fragments versus only 5% observed with EcoRI/MseI (data not shown). However, when genotype analysis was performed on the presence/absence of a band,

the AFLP profiles obtained clearly Rutecarpine indicated very high similarity, with all isolates grouped within a similarity index of 0.97. This genetic variability is much lower than what we have observed for the species C. metapsilosis and C. orthopsilosis, for which we observed a greater number of polymorphic bands [16, 17]. Our results are in agreement with the observation that the frequency of single nucleotide polymorphisms (SNPs) in C. parapsilosis is 30 to 70 fold lower than in other Candida species [30]. The low level of variability found suggests a clonal or selfing strategy of reproduction, supporting the hypothesis of a successful species recently emerged as a genetically homogeneous population diverged from a common ancestor [31].