) glass liner, in the case of SPME analysis. Split injection mode was used (154▒mL/min). The GC area data were used as an approach to estimate the relative content of farnesol. In this study, O/W/O multiple emulsions have been investigated using various chemical compositions, which include the use of retinol or oleic acid as vehicles for farnesol encapsulation. The prepared multiple emulsions were then employed as soft organic templates to prepare silica capsules by a sol–gel

method involving the hydrolysis and condensation of silane oligomers derived from TEOS used as precursor. The hydrolysis and condensation reactions take place in the water phase though TEOS was previously added to the oil phase (n-decyl alcohol). This is because vigorous stirring MG-132 solubility dmso of the external oil phase facilitate the penetration of TEOS through the surfactant layer surrounding the water phase in which the hydrolysis occur. As the hydrolysis Angiogenesis inhibitor proceed, the water-soluble silica oligomers are

kept inside the aqueous droplet [ 1, 8, 9]. Thus the aqueous phase acts as space-limiting micro-reactors for the hydrolysis process, and the internal oil droplets serve as templates for cores. The use of multiple emulsions in materials synthesis requires judicious control over several experimental parameters in order to achieve emulsion stability. In order to obtain suitable emulsions for the encapsulation of farnesol, several concentrations for Tween 20 and Span 80 were investigated and here results are presented among those that result in the more morphological uniform droplets as evaluated by optical microscopy. Moreover, the droplets average size and size distribution have a major role in the emulsion stability in a way that emulsions with precisely controlled droplet size exhibit better stability. As the interfacial curvature of the internal droplets is oxyclozanide tensed due to the small size of the droplets, the addition of surfactant in the external phase will help the formation of a hole in the external film when the internal drops are

close to the surface. This enables a decrease of the curvature tension that becomes more positive and therefore entropically favorable. In this regards, additional stabilizers such as HPC, PEG, PVP and P123 were used in this study to improve the stability of both external oil and water phases as reported elsewhere [19]. Fig. 1 shows an optical microphotograph of typical O/W/O multiple emulsions employed in this study. These emulsions were used immediately after their preparation. Because TEOS was added to O/W/O emulsions as precursor of the sol–gel method, amorphous SiO2 forms through a series of hydrolysis and condensation reactions involving oligomeric silane species at the O/W interphase. The literature reports attempts to explain the mechanism of formation of SiO2 via the sol–gel method using microemulsions as nanoreactors [1].