At the systemic level, serum IgA peaked around 3 weeks post-infection (WPI) and decreased thereafter (Figure 3). Serum IgG quickly increased to a asymptote around three WPI and remained consistently high throughout the infection (Figure 3). Changes in serum IgA and IgG were significantly different between treatment

(infected and controls) and the interaction between treatment and WPI, MI-503 datasheet when the analysis was corrected for the random effect of the host and the nonindependence of sampling the same individual over time (Table 4). Mucus IgA and IgG patterns exhibited similar trends: values were significantly higher in the infected, compared to controls, and decreased from section 1 to section 4 of the small intestine; mucus IgG also increased with sampling time in infected rabbits (Table 4). Graphidium strigosum: Infected rabbits mounted a strong somatic IgA and IgG response at the systemic level but the local antibody response was relatively low to both adult and L3 stages (Figures 4 and S2).

Specifically, serum IgA and IgG significantly differed between treatments and increased with infection time in infected individuals (Table 5). Mucus IgA and IgG were higher in the infected compared to the controls, and Tigecycline in vivo for the infected, values increased with the course of infection showing stronger response in the fundus compared to the antrum section of the stomach (Table 5). Together these findings suggest that rabbits develop an effective systemic and local antibody response to T. retortaeformis but an inefficient mucosal response to G. strigosum. Trichostrongylus Phosphoglycerate kinase retortaeformis: Total white blood cell

and lymphocyte counts were significantly higher in infected hosts compared to the controls and consistently increased over the course of the infection (Figure 5). No significant trend was recorded for eosinophils and neutrophils, corrected for the random effect of the host and the dependence of sampling the same individual over time (Figure 5). However, a more detailed analysis showed that during the second-to-fifth WPI, coinciding with the peak in antibody response, a strong eosinophilia, anaemia (haemoglobin) and high total white blood cells were recorded in infected compared to control rabbits (Table 6). Graphidium strigosum: A consistent increase in the concentration of eosinophils, lymphocytes, total white blood cells and haemoglobin was observed with the progression of the experiment but no significant differences were recorded between the infected and the controls (Figure 6, Table 6). In line with T. retortaeformis infection high eosinophilia, neutrophilia and total white blood cell concentrations were found during the second-to-the fourth WPI in infected compared to the controls; no significant development of anaemia was observed during this infection.