32 mu g/ml and 2.81 mu g/ml, respectively. Their activity was similar to the activity of cis-platin, which is clinically used as anticancer drug in the treatment of human solid tumours. We also perform quantum chemical calculation of interaction and binding energies in complexes of model systems and 3e with DNA bases. Interaction of real drug 3e with guanine is much stronger than with the remaining nucleobases, and the strongest among all investigated complexes. Computer simulations were performed with ATP-binding domain and DNA-binding R406 site
of hTopoII. Compounds 3a-h were recognized as potential inhibitors of hTopoII.”
“High-speed terrestrial locomotion inevitably involves high acceleration and extensive loadings on the legs. This imposes a challenging trade-off between weight and strength in leg design. This paper introduces a new design paradigm for a robotic leg inspired by musculoskeletal structures. The central hypothesis is that employing a tendon-bone
co-location architecture not only provides compliance in the leg, but can also reduce bone stresses caused by bending on structures. This hypothesis is applied to a leg design, and verified by simulations and the experiments on a prototype. In addition, we also present an optimization scheme to maximize the strength to weight ratio. Using the tendon-bone co-location architecture, the stress on the bone during a stride is reduced by up to 59%. A new foam-core prototyping technique enables creating structural characteristics similar to mammalian bones in the robotic leg. This method allows us to use lighter polymeric CYT387 structures that are cheaper and quicker to fabricate than conventional fabrication methods, and can eventually greatly shorten the design iteration cycle time.”
“The structural stability of guanine quadruplexes depends critically on an unusual configuration of dehydrated Na+ or K+ ions, closely spaced along the central axis of the quadruplex. Crystallography and MDV3100 concentration NMR spectroscopy indicate that these internal ions can be located between the G-quartet planes as well as in the thymine
loops, but the precise ion coordination has been firmly established in only a few cases. Here, we examine the bimolecular diagonal-looped foldback quadruplexes [d(G(3)T(4)G(3))](2) (Q3) and [d(G(4)T(4)G(4))](2) (Q4) by H-2, O-17, and Na-23 magnetic relaxation dispersion (MRD). The MRD data indicate that both quadruplexes contain Na+ ions between the T-4 loops and the terminal G-quartets and that these ions have one water ligand. These ions exchange with external ions on a time scale of 10-60, mu s at 27 degrees C, while their highly ordered water ligands have residence times in the range 10(-8)-10(-6) s. The MRD data indicate that Q4 contains three Na+ ions in the stem sites, in agreement with previous solid-state 23Na NMR findings but contrary to the only crystal structure of this quadruplex.