, 2008), indicating the advantage of MLSA as a good substitute for DNA–DNA hybridization in describing new Vibrio species. The determination of the G+C content of strain MSSRF38T yielded 45.4 mol%, which was in good agreement with the values for the genus Vibrio (Baumann et al., 1984). The strain MSSRF38T had the main phenotypic features of the genus Vibrio; the cells were straight to slightly curved rods, motile,

facultatively anaerobic, Gram-negative, catalase-positive and no growth occurred in the absence of NaCl. These features indicate that the click here strain is probably a species of the genus Vibrio (Baumann et al., 1984). The strain MSSRF38T produced nondiffusible, cellular red pigments, regardless of the presence of light. Acetone/methanol extracts of the red pigments showed maximal absorption at about 535 nm, which is identical to the

absorption spectrum of prodigiosin (Allen, 1967). The phenotypic characteristics of strain MSSRF38T are given in the species description below. Strain MSSRF38T is phenotypically very similar to V. rhizosphaerae DSM 18581T and V. ruber DSM 16370T. It was found earlier that several vibrios have very similar phenotypic features (Gomez et al., 2004; Thompson et al., 2004), and the techniques that are essential for reliable species identification in the genus Vibrio are based on genomic data (Thompson et al., 2004). Table 2 presents the characteristics that differentiate MSSRF38T from its phylogenetically most closely related neighbours. Furthermore, the new species could be differentiated from any other Vibrio species by the following combination of properties: Protein Tyrosine Kinase inhibitor positive for red pigment, gas production from glucose, utilization of d-arabinose, lactose and xylose, no growth in TCBS, negative for oxidase, arginine dihydrolase and ornithine

decarboxylase, and resistant to O/129. The FAME analyses showed that strain MSSRF38T had the main chemotaxonomic features of the genus Vibrio (Lambert et al., 1983; Bertone et al., 1996). The most abundant fatty acids are summed feature 3 (26.2%; comprising C16:1ω7c and/or C15:0 iso 2-OH), C16:0 (20.9%), C18:1ω7c (18.0%), C14:0 (8.4%), C12:0 (6.8%), summed feature 2 (6.5%; comprising an unidentified fatty acid with an equivalent chain length of 10.928 and/or C12:0 ALDE, C16:1 iso I and/or C14:0 3-OH), C12:0 3-OH (6.7%). The following fatty acids filipin were detected in small amounts: C10:0 (0.2%), C10:0 3-OH (0.4%), C14:0 iso (0.2%), C14:0 iso 3-OH (0.2%), C16:0 iso (0.4%), C12:0 2-OH (0.2%), C12:1 3-OH (2.0%), C16:1ω5c (0.1%), C17:0 (0.4%), C18:0 (0.5%), summed feature 1 (0.3%; comprising C13:0 3-OH and/or C15:1 iso I/H), unidentified fatty acid with an equivalent chain length of 12.484 (0.9%), unidentified fatty acid with an equivalent chain length of 11.799 (0.9%). In conclusion, the results of the present study indicate that isolate MSSRF38T should be classified in a novel species of the genus Vibrio, for which the name Vibrio mangrovi sp. nov. is proposed. Vibrio mangrovi (man.