2 Cooked dishes (16), Pork (28), Diary products (14), Beef (6), Seafood (5), Egg products (5), Vegetables (3), Unknown (13). A set of control strains was used to validate the STM GeneDisc® array (Table 3). Reference strain LT2 was used as a positive control for testing SPI genetic markers (ssaQ, mgtC, spi4-D and sopB genes), and virulence plasmid pSLT (spvC gene). Typhimurium strain 08CEB5766SAL was used as a negative control for testing the ssaQ, sopB and spvC markers, whereas the

00-01041 strain kindly provided by the Federal Institute for Risk Assessment (BfR) in Berlin, Germany, was used as a negative template to test {Selleck Anti-diabetic Compound Library|Selleck Antidiabetic Compound Library|Selleck Anti-diabetic Compound Library|Selleck Antidiabetic Compound Library|Selleckchem Anti-diabetic Compound Library|Selleckchem Antidiabetic Compound Library|Selleckchem Anti-diabetic Compound Library|Selleckchem Antidiabetic Compound Library|Anti-diabetic Compound Library|Antidiabetic Compound Library|Anti-diabetic Compound Library|Antidiabetic Compound Library|Anti-diabetic Compound Library|Antidiabetic Compound Library|Anti-diabetic Compound Library|Antidiabetic Compound Library|Anti-diabetic Compound Library|Antidiabetic Compound Library|Anti-diabetic Compound Library|Antidiabetic Compound Library|Anti-diabetic Compound Library|Antidiabetic Compound Library|Anti-diabetic Compound Library|Antidiabetic Compound Library|Anti-diabetic Compound Library|Antidiabetic Compound Library|buy Anti-diabetic Compound Library|Anti-diabetic Compound Library ic50|Anti-diabetic Compound Library price|Anti-diabetic Compound Library cost|Anti-diabetic Compound Library solubility dmso|Anti-diabetic Compound Library purchase|Anti-diabetic Compound Library manufacturer|Anti-diabetic Compound Library research buy|Anti-diabetic Compound Library order|Anti-diabetic Compound Library mouse|Anti-diabetic Compound Library chemical structure|Anti-diabetic Compound Library mw|Anti-diabetic Compound Library molecular weight|Anti-diabetic Compound Library datasheet|Anti-diabetic Compound Library supplier|Anti-diabetic Compound Library in vitro|Anti-diabetic Compound Library cell line|Anti-diabetic Compound Library concentration|Anti-diabetic Compound Library nmr|Anti-diabetic Compound Library in vivo|Anti-diabetic Compound Library clinical trial|Anti-diabetic Compound Library cell assay|Anti-diabetic Compound Library screening|Anti-diabetic Compound Library high throughput|buy Antidiabetic Compound Library|Antidiabetic Compound Library ic50|Antidiabetic Compound Library price|Antidiabetic Compound Library cost|Antidiabetic Compound Library solubility dmso|Antidiabetic Compound Library purchase|Antidiabetic Compound Library manufacturer|Antidiabetic Compound Library research buy|Antidiabetic Compound Library order|Antidiabetic Compound Library chemical structure|Antidiabetic Compound Library datasheet|Antidiabetic Compound Library supplier|Antidiabetic Compound Library in vitro|Antidiabetic Compound Library cell line|Antidiabetic Compound Library concentration|Antidiabetic Compound Library clinical trial|Antidiabetic Compound Library cell assay|Antidiabetic Compound Library screening|Antidiabetic Compound Library high throughput|Anti-diabetic Compound high throughput screening| the spi4_D and mgtC markers. All these negative control strains had been tested BV-6 previously using conventional PCR. Table 3 Set of control strains Strain Source DT104 16S- 23S

spacer ssaQ mgtC spi4_D sopB spvC SGI1 left Junction intI1 bla TEM sul1 LT2   – + + + + + – - – - 05CEB1571SAL ANSES + + + + + + + + – - 07CEB5289SAL ANSES – + + + + – - + + + 07CEB9150SAL ANSES + + + + + – - – + – 01CEB12158 ANSES – + + + + – - – - – 08CEB5766SAL ANSES + – + + – - – - – - 63.48 DTU Food + + + + + – - – + – 61.12 DTU Food – + + + + – - + + + 00-01041 BfR     – -             The specificity of the phage https://www.selleckchem.com/products/gant61.html type DT104 marker targeting the 16S-23S rRNA intergenic spacer region was tested with 43 strains of different phage types: atypical DT146 (n = 1), DT120 (n = 10), DT135 (n = 1), DT99 (n = 1), DT8 Diflunisal (n = 2), DT193 (n = 4), DT30 (n = 2), DT12 (n = 2), DT4 variant (n = 1), U302 (n = 12), DT2 (n = 1), DT208 (n = 1), DT12a (n = 1), DT136 (n = 1), DT18 (n = 1), DT36 (n = 1), U311 (n = 1) and 59 strains of phage type DT104. Phage-typing had already

been performed either in the Laboratory of Gastrointestinal Pathogens at the Health Protection Agency (HPA, London, UK) or in the National Reference Centre on Salmonella at the Institut Pasteur (Paris, France). The presence of SGI1 was explored by targeting the left junction sequence and detecting integrase of class 1 integron gene (intI1) and a sulfonamide resistance determinant (sul1). The positive control strain used for these three markers was S. Typhimurium strain 05CEB1571SAL, a strain isolated from turkey and well-characterized by a European project. Positive results had already been detected for the left junction sequence, intI1 and sul1 genes.